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    Protective effect of histidine on para-nonylphenol-enhanced hydroxyl free radical generation induced by 1-methyl-4-phenylpyridinium ion (MPP+) in rat striatum

    机译:组氨酸对1-甲基-4-苯基吡啶鎓离子(MPP +)诱导的大鼠纹状体中对壬基酚增强羟基自由基生成的?;ぷ饔?

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    The present study examined the antioxidant effect of histidine, a singlet oxygen (~1O_2) scavenger, on para-nonylphenol (an environmental estrogen-like chemical)-enhanced hydroxyl radical (OH) generation induced by 1-methyl-4-phenylpyridinium ion (MPP) in extracellular fluid of rat striatum. Rats were anesthetized, and sodium salicylate in Ringer's solution (0.5 nmol/μl/min) was infused through a microdialysis probe to detect the generation of OH as reflected by the non-enzymatic formation of 2,3-dihydroxybenzoic acid (DHBA) in the striatum. Introduction of para-nonylphenol (10 μM) significantly enhanced MPP~+-induced OH generation. Histidine (25 mM) decreased the para-nonylphenol-enhanced OH formation. Although the level of MPP~+-induced OH formation trapped as DHBA after para-nonylphenol treatment increased, para-nonylphenol failed to increase either the level of dopamine and DHBA formation in the reserpinized animals. These results indicate that para-nonylphenol and MPP~+-enhanced OH generation was based on ~1O_2 production,and histidine may have a preventive effect on para-nonylphenol and MPP~+-induced OH generation in rat striatum.
    机译:本研究研究了组氨酸(单线态氧(?1O_2)清除剂)对由1-甲基-4-苯基吡啶鎓离子诱导的对壬基苯酚(一种环境雌激素样化学物质)增强的羟基自由基(OH)的抗氧化作用。大鼠纹状体细胞外液中的MPP)。麻醉大鼠,并通过微渗析探针将林格氏液中的水杨酸钠(0.5 nmol /μl/ min)注入体内,以检测OH的生成,这是通过非酶法形成的2,3-二羟基苯甲酸(DHBA)反映出来的。纹状体。对壬基苯酚(10μM)的引入显着增强了MPP?+诱导的OH生成。组氨酸(25 mM)减少了对壬基酚增强的OH的形成。尽管在对壬基苯酚处理后,MPP?+诱导的OH形成水平以DHBA的形式被捕获,但对壬基苯酚未能使再固定化动物的多巴胺和DHBA形成水平增加。这些结果表明对壬基酚和MPP?+增强的OH生成是基于?1O_2的产生,而组氨酸可能对大鼠纹状体中对壬基酚和MPP?+诱导的OH产生具有预防作用。

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