• <object id="wmooc"><u id="wmooc"></u></object>
  • 【24h】

    A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax

    机译:从鲈鱼Dicentrarchus labrax分离并鉴定的血清岩藻毒素

    获取原文
    获取原文并翻译 | 示例

    优酷会员7折

    包量

    摘要

    A lectin specific for fucose and galactose was isolated by affinity chromatography on Sepharose CL-6B from the serum of Dicentrarchus labrax. The hemagglutinating activity against rabbit erythrocytes was calcium-independent, and reached its maximum at 37 ℃. Two protein components were found in the hemagglutinating fractions eluted from the Sepharose column. Only the 34 kDa component (DIL2) eluted from the polyacrylamide gels (SDS-PAGE) showed agglutinating activity against rabbit erythrocytes. SDS-PAGE, in non-reducing conditions, revealed a single 66 kDa protein that reacted with antibodies to the 34 kDa component. Therefore, a dimeric structure stabilized by disulfide bonds can be proposed. The Ca~(2+)-independent fucose-binding specificity, a significant amino acid sequence homology of the N-terminal trait, and cross-reaction of eel fucolectin with antibodies to DLL2 suggest that this lectin may be included in the recently identified fucolectin family.
    机译:在琼脂糖凝胶CL-6B上通过亲和色谱法从Dicentrarchus labrax的血清中分离出对岩藻糖和半乳糖具有特异性的凝集素。对兔红细胞的血凝活性与钙无关,在37℃达到最大值。从琼脂糖凝胶柱洗脱的血凝馏分中发现了两种蛋白质成分。从聚丙烯酰胺凝胶(SDS-PAGE)洗脱的只有34 kDa组分(DIL2)显示出对兔红细胞的凝集活性。在非还原条件下,SDS-PAGE显示单个66 kDa蛋白质与34 kDa成分的抗体反应。因此,可以提出通过二硫键稳定的二聚结构。 Ca?(2+)独立的岩藻糖结合特异性,N端性状的显着氨基酸序列同源性以及鳗鱼岩藻糖蛋白与DLL2抗体的交叉反应表明该凝集素可能包含在最近鉴定的岩藻糖蛋白中家庭。

    著录项

    站内服务
    • 论文查重
    • 论文收录引证报告
    • 文档翻译
    • 文档转换
    获取原文

    联系方式:18141920177 (微信同号)

    客服邮箱:kefu@zhangqiaokeyan.com

    京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司?版权所有
    • 客服微信

    • 服务号

    婷婷五月亚洲综合图区_五十路A片熟妇在线观看_国产成人免费资源在线播放_欧美成人熟妇激情视频