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    Comparative study of blood group-recognizing lectins toward ABO blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides

    机译:新糖蛋白,糖蛋白和复杂型寡糖上针对ABO血型抗原的血型识别凝集素的比较研究

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    Binding specificities of ABO blood group-recognizing lectins toward blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides were studied by lectin-blotting analysis, enzyme linked immunosorbent assay and lectin-conjugated agarose column chromatography. Human serum albumin conjugated with A- and B-trisaccharides was clearly recognized by Helix pomatia (HPA), Phaseolus lunatus, Dolichos biflorus agglutinins, and Griffonia simplicifolia I agglutinin B_4, respectively, Almost the same results were obtained for human group A and B ovarian cyst and A-active hog gastric mucins, but Glycine max agglutinin only reacted to the group A hog mucin. when human plasma von willebrand factor (vWF), having Asn-linked blood group antigens, was tested, HPA was highly sensitive to blood group A antigen on the vWF. Ulex europaeus agglutinin I (UEA-I) preferentially bound to the vWF from blood group O plasma. Within the GalNAc-recognizing lectins examined, a biantennary complex-type oligosaccharide having the blood group A structure retarded on an HPA-agarose column, and the affinity was diminished after digestion with α-N-acetylgalactosaminidase. This product bound to UEA-I agarose column. These results indicate that HPA and UEA-I are most sensitive for detection of glycoproteins possessing small amounts of blood group A and H antigens and also useful for fractionation of complex-type oligosaccharides with blood group A and H antigens, respectively.
    机译:通过凝集素印迹分析,酶联免疫吸附测定和凝集素缀合的琼脂糖柱色谱法研究了ABO血型识别凝集素对新糖蛋白,糖蛋白和复合型寡糖上血型抗原的结合特异性。 Helix pomatia(HPA),Phaseolus lunatus,Dolichos biflorus a凝集素和Griffonia simplicifolia I凝集素B_4分别清楚地识别了与A-和B-三糖缀合的人血清白蛋白,对于人A和B卵巢组几乎获得了相同的结果囊肿和A型活性猪胃粘蛋白,但Glycine max凝集素仅与A组猪粘蛋白反应。当测试具有Asn连锁血型抗原的人血浆血管性假血友病因子(vWF)时,HPA对vWF上的A型血抗原高度敏感。欧洲油菜凝集素I(UEA-I)优先与血型O血浆中的vWF结合。在所检查的GalNAc识别性凝集素中,在HPA-琼脂糖柱上具有血型A结构的双天线复合型寡糖被延迟,并且在用α-N-乙酰半乳糖苷酶消化后亲和力降低。该产物结合到UEA-1琼脂糖柱上。这些结果表明,HPA和UEA-1对于具有少量血液A和H抗原的糖蛋白的检测最敏感,并且还分别用于分离具有血液A和H抗原的复合型寡糖。

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