摘要：选用复合蛋白酶酶解扇贝边制备水解液,并优化酶解工艺条件;采用中空纤维超滤膜对水解液进行分离纯化,制造高质量的水解动物蛋白,并测试膜分离试验操作条件对分离效率的影响.研究结果表明,先用枯草杆菌蛋白酶酶解4 h,然后用风味蛋白酶继续酶解,可以显著提高酶解液中多肽、短肽及游离氨基酸的含量;超过滤的操作压力、温度、酶解液的pH值及浓缩比等因素都将影响超过滤效率,在操作压力不高于0.10 MPa,pH值为7.0～7.5,温度为43℃,每过滤10 min后就对超过滤膜进行2 min的反冲洗等操作条件下,可以大大提高超过滤的分离效率、减少膜的污染及延长膜的使用寿命;试验结果还表明,超过滤分离后水解液的感官指标明显优于未分离的水解原液.%After hydrolysis with mixed protease, the hydrolysates of scallop skirt was purified by hollow fi-ber ultrafiltration(UF). The processing parameters for the manufacture of liquid-HAP in good quality wereinvestigated. Results show that the content of polypeptides, peptides, and free amino acids in the liquid-HAPis largely increased by using flavorzyme after 4 h pre-hydrolysis with A. S. 1. 398 neutral subtilisin. And thatpermeation flux was affected by operating pressure, temperature, pH value of hydrolysates, and the ratio ofvolume to concentration (RVC). Optimal operative condition, which might prolong working time of the de-vice with high permeation flux, minimize pollution of membranes and raise separating efficiency, is pH 7.0～ 7.5 at 43 ℃ under 0.10 MPa and the membranes rinsed repeatedly for 2 minutes after every operating for10 minutes. These results suggest that sense index of fractionated hydrolysates is superior to that of the origi-nal hydrolysates.
摘要：An integrated creative theory framework is proposed in this paper, and then a replacement-basedcreative approach is introduced based on this theory. The approach employs a hyper-graph rewriting system toorganize intrinsic information of the system and to model creativity process. It takes an existing system ratherthan external requirements as the starting point, and then an innovative structure involving a new system canbe obtained by a sequence of formal operations on the initial hyper-graph structure of the system.%探讨创新设计的理论框架,进而提出一种基于系统内部信息置换的创新设计方法.该方法利用超图重写系统组织系统的内部结构和创新过程.它以现有系统而不是外部需求为创新的起点,通过对现有系统的超图进行系列置换而产生新的创造.
摘要：从设计方法的角度讨论了行星齿轮传动CAD系统的合理结构和内容.介绍了优化设计模型,采用体积和模数同时极小化的设计目标,提出了分层优化策略;对不同的变量采用不同的目标函数,可使计算效率提高,优化结果的全局最优性和可靠性得到保证.同时从实际设计需求出发提出了程序运行的控制方法.%Rational structure and content of CAD system for the planetary gear transmission are discussedfrom the point of design method. The model of optimum design is introduced. The design objective of mini-mizing volume and module simultaneously are adopted, and a graded optimum strategy is presented. Diffe-rent objective functions were used for the first time in accordance with different variables. As a result effi-ciency of calculation is raised, optimization of an overall situation and reliability of an optimum solution areensured. Besides, the control methods of the program are put forward as a requirement of actual design.
摘要：A novel and sensitive thin-layer chromatography(TLC) method for specific and simultaneous de-tection of both lactone form Monacolin K and acid form Monacolin K is developed in this study. The TLCworks in the following conditions: The TLC plate was Silica Gel 60, RP - 18 F254, 0.5 mm, chemicallymodified layer, Merck, Germany; The solvent system was chloroform: methanol: ammonium hydroxide =25 : 3 : 1 (volume ratio); Monitoring the TLC under 365 nm after heating it with a slight coating of thecharring reagent 30% (volume fraction) H2 SO4 ethanol on the TLC plate, allowed the specific detection ofMonacolin K and the low detection limit was first time brought down to 30 ng. The present TLC method issuitable for the routine qualitative and semi-quantitative analysis of the bulk materials containingMonacolin K.%通过实验筛选出了适于特异性的同步检测酸式和内酯式莫奈呵啉K的薄层色谱法(TLC).最佳薄层色谱条件为:薄层板采用德国Merck公司的化学修饰薄层板Silica Gel 60,RP-18 F254,0.5 mm;展开剂系统采用V(氯仿):V(甲醇):V(氨水)=25:3:1;炭化试剂采用30%(体积分数)的硫酸乙醇溶液;紫外检测波长为365 nm.该法实现了酸式和内脂式莫奈呵啉K的检测特异性与同步性,并将检测灵敏度提高到绝对检测量30 ng.该法适用于任何含莫奈呵啉K的样品的检测,特别适用于大批量经常性的定性或半定量莫奈呵啉K的检测.